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Dextran Sulfate Sodium (DSS)-Induced Colitis Model

Inflammatory bowel diseases (IBDs) are chronic, relapsing inflammatory condition of the gastrointestinal tract, primarily including ulcerative colitis (UC) and Crohn’s disease (CD). Etiologic theories of IBD commonly involve heredity, dysbiosis, defective mucosal barrier function and aberrant immunoregulation.

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DSS-induced colitis, closely resembles human UC, is one of the well-established animal models of mucosal inflammation that has been widely used in the study of IBD pathogenesis and preclinical studies. The duration and severity of DSS model are controllable to meet different study needs. 

NHP model provides advantage of closer phylogenetic affinity and cross-reactivity to the human specific biological molecules. It emerges as superior evaluation system to help generate human translational data.


DSS-Induced Colitis in Cynomolgus Monkey

Animal: Cynomolgus monkeys, male

Material: : DSS (Dextran sulfate sodium)

Clinical Readouts of A Small Case (n=2 for Each Group)

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Colonic Histopathology

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Scoring System for Tissue Damage

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Blood Cell Counts

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Whole Blood Cell Counts Demonstrated: 

• Up-Regulated Activity of Innate Immune Response:

        Increased number of phagocytes (monocytes and neutrophils), which can be accounted by the defective intestinal barrier and microbial infection after DSS challenge.

• Slight Anemia:

        RBC& HGB drop was in line with the intestinal bleeding since Day7.

• Rising Platelet Count

        A possible compensatory response to counteract intestinal bleeding.


Clinical Signs

Summary: 

• Progressive development: reduced food intake, body weight loss, diarrheal, hemoccult and rectal bleeding. 

• Abnormal signs in gross anatomy were largely restricted to colon. 

• Shortening of colon length, increasing in colon thickness, colon ulceration and bleeding, epithelial erosion and shedding, enlargement of mesenteric lymph nodes 

• Considerable Reproducibility

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