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Tetanus Toxoid (TTx)-Induced Delayed Type Hypersensitivity (DTH) Model

DTH is a classic model of Type IV hypersensitivity reactions driven by an antigen specific T cell response resulting in inflammation and tissue injury. In preclinical studies, DTH models are widely used for the evaluation of novel immunosuppressive and immunomodulatory agents, as well as in vaccine development.

Case Study:TTx-Induced DTH in Cynomolgus Monkey

Animal: Cynomolgus monkeys, 2.5 - 3.3 kg 

Material: Tetanus Toxoid (TTx) Vaccine


DTH Response in Cynomolgus Monkey

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DTH expressed as skin induration on the challenged spots and treatment with topical Dex or systemic cmpd-X attenuated this response.    


Skin Erythema, Edema, Indurations

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Skin Histopathology Evaluation


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IHC: Immune Cell Infiltrate Determination: Examination

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Monocytes, B &T Cells, and NK Cells in Peripheral Blood

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Similar to Dexamethasone, the cmpd-X treatment group showed a decrease in CD14+ monocyte and classical monocyte (CD14+CD16-). However, levels of activated monocyte (CD14+CD16+) and other major immune cell populations, e.g. B cells, T cells, NK cells, were not affected as compared to vehicle group.    




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