Sample Tumor Models - PharmaLegacy

ONCOLOGY

Oncology Service Platform

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Sample Tumor Models

Sample Tumor Models Oncology

Colorectal Carcinoma

Research Models:

Nude mice:

Subcutaneous xenograft models (HCT-116, T-29, COLO205, HCT-15)
Orthotopic implantation models
Human primary CRC models (7 tumors, subcutaneous or orthotopic xenografts )
Luciferase-reporter based CRC imaging model (orthotopic growth and metastasis, HT-29-Luc)

Study Readouts:

Tumor biology, biomarkers, and molecular pharmacology

Tumor volume/weight, animal body weight and mortality
Cytokine/chemokine/biomarker analysis (ELISA, Q-PCR, Western blot)
Kinase activity assay and phosphorylation analysis
Hematology

Tumor histopathology

Histology: morphology and differentiation
Immunohistochemistry: proliferation, apoptosis, angiogenesis and tumor markers

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Gastric Carcinoma

Research Models:

Nude mice:

Subcutaneous xenograft models (MKN-45, NCI-N87, and AGS)
Orthotopic implantation models
Human primary GC models (subcutaneous or orthotopic xenografts)
Luciferase-reporter based GC imaging model (MKN-45-Luc, orthotopic growth and metastasis)

Study Readouts:

Tumor biology, biomarkers, and molecular pharmacology

Tumor volume/weight, animal body weight and mortality
Cytokine/chemokine/biomarker analysis (ELISA, Q-PCR, Western blot)
Drug target activities: kinase activity assay and phosphorylation analysis
Drug target expression (ELISA, Western blot, RT-PCR)
Cell based assays (MTT, apoptosis, and invasion in culture)

Tumor histopathology

Histology: morphology and differentiation
Immunohistochemistry: proliferation, apoptosis, angiogenesis and tumor markers

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Hepatocellular Carcinoma

Research Models:

Nude mice:

Human HCC s.c. xenograft model (> 10 tumor cells lines, > 5 Asia HCC lines)
Human HCC orthotopic implantation models (Liver orthotopic modeling)
Human primary tumor derived HCC models in nude mice (representing human HCC)
Luciferase-reporter based HCC imaging model (growth and metastasis following orthotopic implantation)

Nude rats:

Human HCC s.c. xenograft model (Combinational study of PK-PD and local drug delivery)
Human HCC orthotopic implantation models

Study Readouts:

Tumor biology, biomarkers, and molecular pharmacology

Tumor volume/weight, animal body weight and mortality
Hematology and liver chemistry
Cytokine/chemokine/biomarker analysis (ELISA, Q-PCR, Western blot)
Drug target activities in liver/tumor (kinase activity and phosphorylation analysis)
Drug target expression in liver/tumor (RT-PCR, Western blot)

Tumor histopathology

Histology: morphology and differentiation
Immunohistochemistry: proliferation, apoptosis, angiogenesis and tumor markers

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