PharmaLegacy: Drug Discovery CRO

Quickly identify hits and confidently optimize leads with PharmaLegacy’s range of high fidelity, high throughput in vitro assays.

Accelerate Your Early-Stage Pipelines

High fidelity assays performed to exacting standards are crucial for you to identify those rare, valuable compounds with the potential to ultimately win approval. PharmaLegacy has the assays and experience to screen your libraries and unearth the key insights that you need to find those winning compounds.

Our highly experienced staff, rigorous assay validation, meticulous protocols, and ability to execute quickly make PharmaLegacy the partner of choice for pharmaceutical companies who can’t afford to compromise but still need to hold to a budget. Join 18 of the top 20 pharmaceutical companies. Optimize your pipelines efficiently and expeditiously with PharmaLegacy.

Our Drug Discovery Services Include:

  • Over 400 kinases & enzymes available
  • Custom assay development with full validation
  • Many classes of kinases and enzymes covered: carboxylases, DNA polymerases, GTPases, Hydrolases, Nicotinamide kinases, Nucleotidases, Oxidases, Phosphatases, Phosphohydrolases, Phosphoribosyltransferases, Proteases, and more
  • Epigenetics Targeted Assays: bromodomain-containing protein binding partner assays, histone acetyltransferase, histone lysine demethylase, histone methyltransferase, protein arginine methyltransferase
  • Full suite of detection technologies: ADP-Glo, AhphaELISA, FI, FP, HTRF, TR-FRET, and more
  • High-throughput, 384-well format
MORE INFO ON KINASE / ENZYME ASSAYS
  • More than 350 BaF3 engineering cell lines
  • Multiple target WT and mutant cell lines
  • Coverage of most kinase families
  • Multiple readouts available (CTG, CCK8, MTT, LDH, and more)
  • Customized cell line generation available
  • HTS-compatible
MORE INFO ON BA/F3 ASSAYS
  • Extensive Cell Line Library: Choose from over 400 pre-characterized GPCR cell lines and more than 50 Ion Channel cell lines.
  • All GPCR subtypes, including Gs, Gq, and Gi
  • Tailored Cell Line Engineering using overexpression, knock out, knock in, knock down, mutation, and report gene integration. Complete cell line validation.
  • High-Throughput Screening in a 384-well assay format
  • Beta Arrestin assays using Nano-BRET/Nano Bit and Enzyme Fragment Complementation (EFC)
  • GPCR internalization assays using HiBit
MORE INFO ON GPCR ASSAYS
  • Over 200 tumor cell lines available, encompassing a diverse range of cancers and genetic backgrounds
  • Drug-resistant cell lines available
  • 2D and 3D cell culture models available
  • Diverse readout options available, including MTT, cck8, CTG, Brdu, and more
  • Flexible treatment times, ranging from acute exposures to chronic dosing (up to 14 days)
  • Combination drug testing
  • High throughput screens, 96-well or 384-well
MORE INFO ON CELL-BASED CYTOTOXICITY ASSAYS
  • T Cell Stimulation / Inhibition Assay
  • T Cell Proliferation Assay
  • Splenocyte Stimulation Assay
  • Macrophage / Whole Blood Stimulation Assay
  • Pro-Inflammatory Stimulation Assay
  • Mixed Lymphocyte Reaction (MLR) Assay
  • Immunogenicity of Recombinant Proteins
MORE INFO ON CELL-BASED IMMUNOMODULATOR ASSAYS
  • Antibody-Dependent Cellular Cytotoxicity (ADCC)
  • Antibody-Dependent Cellular Phagocytosis (ADCP)
  • Complement-Dependent Cellular Cytotoxicity (CDC)
  • T Cell Killing Assay
  • CAR-T / TCR-T Cell Killing Assay
  • CAR-T / TCR-T Safety Assay
MORE INFO ON CELL BASED IMMUNO-ONCOLOGY ASSAYS
  • Antibody Affinity Evaluation
  • Antibody Internalization Assay
  • ADC Cytotoxicity Assay
  • Bystander Killing Assay
  • ADCC and CDC Assay
MORE INFO ON IN VITRO ADC EVALUATION
  • Anti-Virus MRNA vaccine evaluation assays
  • Dendritic cell (DC) vaccine production
  • DC:T re-stimulation assay
  • Immugenicity of neo-antigens
  • Memory T cell subsets after immunization (flow cytometry evaluation)
  • Cytokine expression in T Cell subsets (flow cytometry evaluation)
  • Splenocyte stimulation assays (immunogenicity of IL-2 and IFN-gamma by ELISPOT or FACS)
MORE INFO ON IN VITRO VACCINE EVALUATION
  • Tumour-infiltrating lymphocytes (TILs) analysis
  • Immune profiling assays
  • Antigen binding assays
  • Antigen block assays
  • Cytokine / phospho-protein detection
  • Cytometric bead array (CBA) assays
  • Cell-based function assays (cell cycle, apoptosis, endocytosis, polarization, proliferation, differentiation)
MORE INFO ON FACS ASSAYS
  • hERG Assay
  • Mitochondrial Toxicity (Glu/Gal) Assay
  • Safety Panel (44/90 Panel). This panel screens a broad range of targets involved in drug toxicity in humans, including GPCRs (G-protein coupled receptors), ion channels, nuclear receptors, and transporters.
MORE INFO ON IN VITRO SAFETY ASSAYS
  • QPCR, Digital PCR and RNA-seq
  • Multiplex cytokine measurement by MSD, Luminex, CBA or ELISA
  • TIL (tumor-Infiltrating leukocyte) profiling by FACS: T cells, DCs, Macrophage, NK Monocytes, MDSC, etc
MORE INFO ON BIOMARKERS & PHARMACODYNAMICS ANALYSIS
Contact PharmaLegacy Today!

The Experience to Deliver

PharmaLegacy has supported over 600 IND applications. We have been trusted with critical studies by 18 of the top 20 pharmaceutical companies. Combined with the extensive experience of our scientific staff – over 15 years on average – PharmaLegacy is ready to fill your drug discovery pipelines with promising candidates.

The Tools to Succeed

With 380,000 square feet of facility space, the latest in high-throughput analytical equipment, and a plethora of extensively validated assays, PharmaLegacy is equipped to screen your libraries for all relevant hits and provide the deep, rich data you need to confidently choose your lead compounds.

Take Your Pipeline Further

When you are ready to move your lead compounds into preclinical development, stick with us. PharmaLegacy has over 1,500 animal models available immediately, including on-site non-human primate colonies. Our capacity for over 30,000 animals and models spanning over 100 indications give us the scale and throughput to quickly initiate and execute your studies.

DRUG DISCOVERY DONE RIGHT

    Contact us today to accelerate your drug discovery. See for yourself how you can get world-class study execution using the latest technologies, on budget and with no delay.

    Your drug discovery is too important to compromise. Tell us your study needs and see for yourself why the clients who work with PharmaLegacy stay with PharmaLegacy.